Title: Affymetrix CEL analysis. Description:
This document describes Affymetrix recommended labeling protocols for use with GeneChip Exon Arrays.
This document describes Affymetrix recommended hybridization protocols for use with GeneChip Exon Arrays.
Within each treatment, equal weight of ?ne bark powder from two harvested plants of P. × canescens was pooled
Plantlets of P. × canescens (P. tremula × P. alba)were produced by micropropagation and cultivated in a climate chamber (day/night temperature, 25/18 C; relative air humidity, 50–60%; light per day, 14 h and photosynthetic photon micro ux, 150 umol m–2 s–1). (Parameters: time unit = seconds, temperature unit = C)
Populus x canescens was used and grown in a climate chamber. After 10-week cultivation, six plants were treated with 200 uM CdSO4 for 20 days and other six plants served as the controls.
Total RNA was extracted and purified using RNAqueousTM phenol-free total RNA isolation (Cat#AM1912, Ambion, Austin, TX, US) and Plant RNA Isolation Aid (Cat#AM9690, Ambion, Austin, TX, US) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). (Parameters: Extracted product = total_RNA, Amplification = none)