7 protocols
AccessionNameType
P-AFFY-6
feature_extraction
Title: Affymetrix CEL analysis. Description:
P-AFFY-12
labeling
URL: http://www.affymetrix.com/support/technical/manual.affx
Affy_whole_transcript_labelling.pdf
This document describes Affymetrix recommended labeling protocols for use with GeneChip Exon Arrays.
P-AFFY-13
hybridization
URL: http://www.affymetrix.com/support/technical/manual.affx
Affy_whole_transcript_labelling.pdf
This document describes Affymetrix recommended hybridization protocols for use with GeneChip Exon Arrays.
P-MTAB-28521
pool
Within each treatment, equal weight of ?ne bark powder from two harvested plants of P. × canescens was pooled
P-MTAB-28519
grow
Plantlets of P. × canescens (P. tremula × P. alba)were produced by micropropagation and cultivated in a climate chamber (day/night temperature, 25/18 C; relative air humidity, 50–60%; light per day, 14 h and photosynthetic photon micro ux, 150 umol m–2 s–1). (Parameters: time unit = seconds, temperature unit = C)
P-MTAB-28520
specified_biomaterial_action
Populus x canescens was used and grown in a climate chamber. After 10-week cultivation, six plants were treated with 200 uM CdSO4 for 20 days and other six plants served as the controls.
P-MTAB-28522
nucleic_acid_extraction
Total RNA was extracted and purified using RNAqueousTM phenol-free total RNA isolation (Cat#AM1912, Ambion, Austin, TX, US) and Plant RNA Isolation Aid (Cat#AM9690, Ambion, Austin, TX, US) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). (Parameters: Extracted product = total_RNA, Amplification = none)