6 protocols
AccessionNameType
P-AFFY-6
feature_extraction
Title: Affymetrix CEL analysis. Description:
Affymetrix:Protocol:Hybridization-Unknown
hybridization
Title: Affymetrix Generic Hybridization. Description:
P-AFFY-2
nucleic acid labeling protocol
Affymetrix in vitro transcription labeling
P-MTAB-28148
grow
1. Prepare 0.5x MS media pH5.7 with 0.7% phytoagar

2. Sow approx. 300 sterile seeds on plate

3. Stratify for 4 days at 4oC

4. Place in a growth cabinet set at 21oC short days (8h light, 16 h dark)

5. Harvest plants after 10 days of growth


(Parameters: start time = 10, time unit = days, min temperature = 21, temperature unit = C, media = 0.5xMS)
P-MTAB-28150
nucleic_acid_extraction
1. 300-600 mg Arabidopsis shoot tissue ground under liquid nitrogen 2. RNA extracted using QIAGEN - RNeasy Plant Mini Kit accoring to manufacturers instructions 3. RNA eluted in 50ul EB (Parameters: Extracted product = total_RNA, Amplification = PCR)
P-MTAB-28149
specified_biomaterial_action
1. Plant culture plates opened in a fume hood

2. Samples sprayed with either mock or DEX solutions:

Mock=0.05% Silwet L77/0.1% Ethanol

DEX =0.05% Silwet L77/0.1% Ethanol/10uM DEX

3. Allow plants to dry

4. Replace lid and return to growth chamber for 4h or 8h