E-MEXP-3711 - Transcription profiling by array of H. speiens spleen plasma cells from immune thrombocytopenia purpura (ITP) patients to investigate gene expression profiles

Status
Released on 28 November 2012, last updated on 12 December 2012
Organism
Homo sapiens
Samples (15)
Array (1)
Protocols (5)
Description
Splenic tissues from immune thrombocytopenia purpura (ITP) patients splenectomized after

primary failure of treatment with the B-cell depleting agent rituximab were analyzed, and

antibody-secreting cells were identified as the major B-cell population resisting the treatment.

The phenotype, antibody specificity and gene expression profile of these cells were characterized

and compared to antibody-secreting cells from untreated ITP spleens and from healthy tissues.

Anti-platelet-specific plasma cells (PC) were detected in the spleen of ITP patients up to 6

months after rituximab treatment, and the PC population displayed a long-lived program similar

to the one of bone marrow PC, thus explaining for most of these patients the absence of response

to rituximab and the response to splenectomy. When analyzed by multiplex PCR at the singlecell

level, normal splenic PC showed a markedly different gene expression profile, with an

intermediate signature including genes characteristic of both long-lived PC and proliferating

plasmablasts. Surprisingly, long-lived PC were not detected either in the inflammatory

environment of the untreated ITP spleen. These results suggest that neither the normal nor the

auto-immune splenic environment favors the differentiation and residence of long-lived PC, and

that it is most probably the milieu generated by B-cell depletion that promotes their local

settlement.
Experiment types
transcription profiling by array, cell type comparison, clinical history, co-expression, in vivo
Contact
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-3711.idf.txt
Sample and data relationshipE-MEXP-3711.sdrf.txt
Raw data (1)E-MEXP-3711.raw.1.zip
Array designA-AFFY-44.adf.txt
Links