E-MEXP-3711 - Transcription profiling of plasma cells and plasmablasts from patients with primary immune thrombocytopenia after rituximab treatment compared to treatment naïve patients or healthy donors

Status
Released on 8 August 2013, last updated on 20 October 2015
Organism
Homo sapiens
Samples (15)
Array (1)
Protocols (5)
Description
Splenic tissues from immune thrombocytopenia purpura (ITP) patients splenectomized after primary failure of treatment with the B-cell depleting agent rituximab were analyzed, and antibody-secreting cells were identified as the major B-cell population resisting the treatment. The phenotype, antibody specificity and gene expression profile of these cells were characterized and compared to antibody-secreting cells from untreated ITP spleens and from healthy tissues. Anti-platelet-specific plasma cells (PC) were detected in the spleen of ITP patients up to 6 months after rituximab treatment, and the PC population displayed a long-lived program similar to the one of bone marrow PC, thus explaining for most of these patients the absence of response to rituximab and the response to splenectomy. When analyzed by multiplex PCR at the singlecell level, normal splenic PC showed a markedly different gene expression profile, with an intermediate signature including genes characteristic of both long-lived PC and proliferating plasmablasts. Surprisingly, long-lived PC were not detected either in the inflammatory environment of the untreated ITP spleen. These results suggest that neither the normal nor the auto-immune splenic environment favors the differentiation and residence of long-lived PC, and that it is most probably the milieu generated by B-cell depletion that promotes their local settlement.
Experiment types
transcription profiling by array, cell type comparison, clinical history, co-expression, in vivo
Contact
Citation
B cell depletion in immune thrombocytopenia reveals splenic long-lived plasma cells. Matthieu Mahévas, Pauline Patin, François Huetz, Marc Descatoire, Nicolas Cagnard, Christine Bole-Feysot, Simon Le Gallou, Mehdi Khellaf, Olivier Fain, David Boutboul, Lionel Galicier, Mikael Ebbo, Olivier Lambotte, Mohamed Hamidou, Philippe Bierling, Bertrand Godeau, Marc Michel, Jean-Claude Weill, Claude-Agnès Reynaud. , Europe PMC 23241960
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-3711.idf.txt
Sample and data relationshipE-MEXP-3711.sdrf.txt
Raw data (1)E-MEXP-3711.raw.1.zip
Array designA-AFFY-44.adf.txt
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