E-MEXP-3687 - The dependence on NFAT levels discriminates conventional T cells from Foxp3 expressing regulatory cells
Released on 15 October 2012, last updated on 10 February 2014
In this study, we used microarrays to investigate the gene expression program in conventional T cells, nTreg and conventional T cells treated with TGFbeta (iTreg) from wild-type mice and and mice having NFAT1/NFAT2 double-deficient (DKO) T cells. CD4+CD25- and CD4+CD25+ T cells were isolated by MACS and stimulated for 24 h with anti-CD3 and anti-CD28 antibodies in the absence or presence of TGFbeta. RNA was extracted and microarray analyses were performed. The data represents two independent biological replicates.
transcription profiling by array, all pairs, cell type comparison, co-expression, ex vivo, replicate
Dependence on nuclear factor of activated T-cells (NFAT) levels discriminates conventional T cells from Foxp3+ regulatory T cells. Martin Vaeth;Ulrike Schliesser; Gerd Muller; Sonja Reissig; Kazuki Satoh; Andrea Tuettenberg; Helmut Jonuleit; Ari Waisman; Martin R. Muller; Edgar Serfling; Birgit S. Sawitzki; Friederike Berberich-Siebelt. Proc Natl Acad Sci U S A 109(40):16258-16263 (2012), Europe PMC 22991461