E-MEXP-3661 - Transcription profiling by array of aMHC-Cre-mediated overexpression of N2ICD in mouse to analyze whether Numb/Numblike are involved in inhibition of Notch activity in the developing heart
Last updated on 8 April 2013, released on 9 April 2013
The ventricular wall of the heart is composed of trabeculated and compactlayers, which are separated by yet unknown processes during embryonic development. Notch signaling plays important roles in cardiac development. Mutations in Notch signaling components are related to human congenital heart diseases Two homologues, Numb and Numblike (Numbl), are expressed in mammals, which also act as inhibitors of Notch signaling. Here we investigate the role of Notch2 and Numb/Numblike during myocardial trabeculation and compaction. The aim of the experiment is to analyze whether Numb/Numblike are involved in inhibition of Notch activity in the developing heart. Since Overexpression of N2ICD phenocoped Numb/Numblike mutants, we performed micoarray using total RNA from aMHC-Cre-mediated overexpression of N2ICD.
transcription profiling by array, co-expression, genetic modification, in vivo
Inhibition of Notch2 by Numb/Numblike controls myocardial compaction in the heart. Yang J, Bücker S, Jungblut B, Böttger T, Cinnamon Y, Tchorz J, Müller M, Bettler B, Harvey R, Sun QY, Schneider A, Braun T. Cardiovascular Research 96(2):276-285 (2012)