6 protocols
AccessionNameType
P-AFFY-12
labeling
URL: http://www.affymetrix.com/support/technical/manual.affx
Affy_whole_transcript_labelling.pdf
This document describes Affymetrix recommended labeling protocols for use with GeneChip Exon Arrays.
P-AFFY-13
hybridization
URL: http://www.affymetrix.com/support/technical/manual.affx
Affy_whole_transcript_labelling.pdf
This document describes Affymetrix recommended hybridization protocols for use with GeneChip Exon Arrays.
P-MTAB-26775
image_acquisition
Name: Affymetrix MAS 5.0 Standard Scanning

Width of Image Produced (Pixels): 4733

Height of Image Produced (Pixels): 4733

X Resolution (microns): 3

Y Resolution (microns): 3

Scanner Name: Agilent 2500A GeneArray Scanner

Software Name: Affymetrix Micro Array Suite 5.0


(Parameters: Scanning hardware = HP GeneArray [Affymetrix/HP], Scanning software = Scanning software)
P-MTAB-26774
specified_biomaterial_action
PNT1A and PC3 cells were cultured for 48 h before the culture medium was removed and replaced with fresh medium containing the PI3 Kinase Inhibitor LY294002 (Cat.No.9901, Cell Signaling Technology, Inc.) at the final concentration of 50µM or vehicle DMSO for 1 hour. Cells were subsequently treated with 10µM PY-ITC, 10µM SF, or vehicle DMSO, for 8 hours.
P-MTAB-26773
nucleic_acid_extraction
Total RNA was isolated using the QIAGEN® RNeasy Mini Kit. Quality of the resulting RNA was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies).
(Parameters: Extracted product = total_RNA, Amplification = none)
P-MTAB-26772
grow
PNT1A and PC3 cells were cultured for 48 h before the culture medium was removed and replaced with fresh medium containing the PI3 Kinase Inhibitor LY294002 (Cat.No.9901, Cell Signaling Technology, Inc.) at the final concentration of 50µM or vehicle DMSO for 1 hour. Cells were subsequently treated with 10µM PY-ITC, 10µM SF, or vehicle DMSO, for 8 hours.
(Parameters: time unit = seconds, temperature unit = C)