E-MEXP-3619 - Transcription profiling of S. cerevisiae haploid Sigma1278b strains with msa1 or msa2 or tec1 deletions
Released on 25 October 2013, last updated on 11 December 2014
We determined and classified Msa1-, Msa2- and Tec1-regulated genes in haploid strains of the Sigma1278b genetic background under vegetative growth conditions. For this purpose, we measured transcriptional profiles of five different haploid MATa yeast strains of the following relevant genotypes: (JvdF_94 = YHUM1694) control; (JvdF_64 = YHUM1864) msa1-delta; (JvdF_65 = YHUM1865) msa2-delta; (JvdF_19 = YHUM1900) msa1-delta msa2-delta; (JvdF_17 = YHUM1700) tec1-delta. All strains were grown in duplicate in SC medium lacking leucine and histidine at 30 degrees C to an optical density of 1.0 before extraction of total RNA and transcriptional profiling.
transcription profiling by array, co-expression, replicate, transcript identification
The transcription factors Tec1 and Ste12 interact with coregulators Msa1 and Msa2 to activate adhesion and multicellular development. van der Felden J, Weisser S, Brückner S, Lenz P, Mösch HU. Mol Cell Biol 34(12):2283-2293 (2014), Europe PMC 24732795