E-MEXP-3618 - Transcription profiling by array of human monocytes to study their involement in HIV-Tuberculosis associated Immune Reconstitution Inflammatory Syndrome at the initiation of combined antiretroviral therapy
Last updated on 2 November 2012, released on 1 April 2013
Tuberculosis-associated Immune Reconstitution Inflammatory Syndrome (TB-IRIS) is a common complication in HIV-TB co-infected patients receiving combined antiretroviral therapy (cART). While monocytes/macrophages play major roles in both HIV- and TB-infection individually, a putative contribution of monocytes to the development of TB-IRIS remains unexamined. We performed a genome-wide array analysis on MOs purified from peripheral blood mononuclear cells (PBMCs) obtained before initiation of combined antiretroviral therapy (cART) to verify whether the transcriptome of MOs was already significantly modulated (even before receiving cART) in HIV+/TB+ patients who later developed TB-IRIS compared to control HIV+/TB+ patients who did not develop the complication . The subjects under study included a subset of 18 TB-IRIS patients and controls matched for age, gender and CD4 count.
transcription profiling by array, co-expression, disease state, ex vivo
Modulations of the Complement System in Peripheral Blood Monocytes Contribute to Tuberculosis-associated Immune Reconstitution Inflammatory Syndrome (TB-IRIS).