E-MEXP-3610 - Transcription profiling by array of Saccharomyces cerevisiae (yeast) to analyse and classify Whi3-regulated and ploidy-regulated genes in haploid and diploid strains of the Sigma1278b genetic background under vegetative growth conditions
Released on 19 June 2013, last updated on 11 September 2013
We analyzed and classified Whi3-regulated and ploidy-regulated genes in haploid and diploid strains of the Sigma1278b genetic background under vegetative growth conditions.
For this purpose, we measured transcriptional profiles of two different haploid MATa and one diploid MATa/a yeast strains of the following genotypes: WHI3 strain (SS_YHUM468=YHUM0468), whi3 strain (SS_ySS137=YHUM1920) and whi3-delta/whi3-delta strain (SS_ySS137dipl=YHUM2152). All three strains were grown in duplicate in YNB medium supplemented with tryptophan and uracil at 30 degrees C to an optical density of 1.0 before extraction of total RNA and transcriptional profiling
transcription profiling by array, co-expression, individual genetic characteristics, replicate
The RNA-Binding Protein Whi3 Is a Key Regulator of Developmental Signaling and Ploidy in Saccharomyces cerevisiae. Sarah Schladebeck; Hans-Ulrich Mosch. Genetics 195(1):73, Europe PMC 23770701