E-MEXP-3610 - Transcription profiling by array of Saccharomyces cerevisiae (yeast) to analyse and classify Whi3-regulated and ploidy-regulated genes in haploid and diploid strains of the Sigma1278b genetic background under vegetative growth conditions

Status
Released on 19 June 2013, last updated on 11 September 2013
Organism
Saccharomyces cerevisiae
Samples (6)
Array (1)
Protocols (6)
Description
We analyzed and classified Whi3-regulated and ploidy-regulated genes in haploid and diploid strains of the Sigma1278b genetic background under vegetative growth conditions.

For this purpose, we measured transcriptional profiles of two different haploid MATa and one diploid MATa/a yeast strains of the following genotypes: WHI3 strain (SS_YHUM468=YHUM0468), whi3 strain (SS_ySS137=YHUM1920) and whi3-delta/whi3-delta strain (SS_ySS137dipl=YHUM2152). All three strains were grown in duplicate in YNB medium supplemented with tryptophan and uracil at 30 degrees C to an optical density of 1.0 before extraction of total RNA and transcriptional profiling

Experiment types
transcription profiling by array, co-expression, individual genetic characteristics, replicate
Contact
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-3610.idf.txt
Sample and data relationshipE-MEXP-3610.sdrf.txt
Raw data (1)E-MEXP-3610.raw.1.zip
Processed data (1)E-MEXP-3610.processed.1.zip
Array designA-AFFY-47.adf.txt
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