E-MEXP-3576 - Comparative genomic hybridization by array of yeast to to analyse aneuploidy in mutant strains (ies6d and ino80d) compared to wild-type over time

Status
Last updated on 15 May 2013, released on 16 May 2013
Organism
Saccharomyces cerevisiae
Samples (11)
Array (1)
Protocols (4)
Description
Genomic DNA was collected from ies6d or ino80d after undergoing 40 or 80 generations post-sporulation of IES6/ies6 or 20 or 40 generations post-sporulation of INO80/ino80 heterozygous diploid strains, respectively. (1 day = 10 generations.) Genomic DNA was then amplified, and hybridized to a whole genome tiling microarray. Copy number variation was measured relative to a G1 arrested wild type sample.
Experiment types
comparative genomic hybridization by array, comparative genome hybridization, genetic modification, in vivo, strain or line, time series
Contact
Citation
The INO80 chromatin remodeling complex prevents polyploidy and maintains normal chromatin structure at centromeres. Chambers AL, Ormerod G, Durley SC, Sing TL, Brown GW, Kent NA, Downs JA . Genes & Development :2590–2603 (2012)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-3576.idf.txt
Sample and data relationshipE-MEXP-3576.sdrf.txt
Raw data (2)E-MEXP-3576.raw.1.zip, E-MEXP-3576.raw.2.zip
Array designA-AFFY-42.adf.txt
Links