7 protocols
AccessionNameType
P-AGIL-5
Hybridization
Hyridization was carried out according to manufacturer specifications.
P-MTAB-25367
image_acquisition
The scanning was performed according to the manufacture’s protocol (Agilent technologies, A Method for Quantifying the performance of a DNA Microarray Scanner, Publication Number 5988-9498EN).
(Parameters: Scanning hardware = DNA Microarray Scanner BA [Agilent Technologies], Scanning software = Feature Extraction Software [Agilent])
P-MTAB-25366
labeling
The labeling experiment was performed according to the manufacture's protocol (Agilent technologies, Low RNA Input Fluorescent Linear Amplification Kit Protocol, Version 5.0.1).
(Parameters: Amplification = none, Mass unit = Micro gram)
P-MTAB-25369
labeling
The labeling experiment was performed according to the manufacture's protocol (Agilent technologies, Low RNA Input Fluorescent Linear Amplification Kit Protocol, Version 5.0.1).
(Parameters: Amplification = none, Mass unit = Micro gram)
P-MTAB-25368
nucleic_acid_extraction
Total RNAs were isolated using the RNAiso Reagent (Takara, Japan).
(Parameters: Extracted product = total_RNA, Amplification = none)
P-MTAB-25370
grow
The growth conditions of the plants are as follows: The plants were grown in the plastic dishes (50 plants/plastic dish) containing germination medium (GM). GM contained Murashige and Skoog salts, 1% sucrose (WAKO, Osaka, Japan) and 0.83% Bacto-agar (Difco, Detroit, MI, USA). The plants were grown for 2 weeks in a growth chamber at 22C and 50-60% humidity under 16 hr light/8 hr dark.
(Parameters: time unit = seconds, temperature unit = C)
P-MTAB-25365
specified_biomaterial_action
The plants were incubated at 22C under non-stress condition.