E-MEXP-3497 - Transcription profiling by array of bovine gene expression changes during inflammatory gamma-herpesvirus infection
Released on 26 October 2012, last updated on 16 October 2015
Changes in gene expression in lymph node and kidney of cattle infected with alcelaphine herpesvirus-1. All animals in the group infected with AlHV-1 showed clinical signs of Malignant catarrhal fever (MCF) within one month. Diagnosis of MCF in these animals was confirmed by diagnostic PCR. No clinical signs were seen in the control animals, which were subject to post-mortem examination separately from the MCF-affected animals. RNA samples from kidney and lymph node of 4 MCF-affected and 4 control animals that passed the RNA quality criteria were used for array analysis. Quality assessments of probe-level Affymetrix chip data and normalised data were carried out using statistical and graphical methods including MA plots and smoothed histogram plots. The MA-plots showed normalisation was effective; while smoothed histograms indicated there was no systematic bias in the data.
transcription profiling by array, co-expression, disease state, in vivo
Host gene expression changes in cattle infected with Alcelaphine herpesvirus 1. Russell GC, Benavides J, Grant DM, Todd H, Thomson J, Puri V, Nath M, Haig DM. , Europe PMC 22925730