E-MEXP-3469 - Role of JunD in macrophage activation under LPS stimulation

Status
Released on 1 April 2013, last updated on 4 May 2017
Organism
Rattus norvegicus
Samples (64)
Array (1)
Protocols (9)
Description
To investigate the effects of the differences seen in the JUND binding patterns between LEW, WKY and WKY.LCrgn2 bone marrow derived macrophages (BMDMs), we have 1) analysed gene expression changes over a time course (4 time points: 0,2,4 and 8 hrs) of stimulation with lipopolysaccharide (LPS) using whole transcript expression microarrays, 2) knocked down JunD in WKY BMDMs and analysed gene expression by microarrays.
Experiment types
transcription profiling by array, co-expression, compound treatment, genetic modification, in vitro, reference, time series
Contact
Citations
Combined ChIP-Seq and transcriptome analysis identifies AP-1/JunD as a primary regulator of oxidative stress and IL-1β synthesis in macrophages. Hull RP, Srivastava PK, DSouza Z, Atanur SS, Mechta-Grigoriou F, Game L, Petretto E, Cook HT, Aitman TJ, Behmoaras J. , Europe PMC 23398888
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-3469.idf.txt
Sample and data relationshipE-MEXP-3469.sdrf.txt
Raw data (2)E-MEXP-3469.raw.1.zip, E-MEXP-3469.raw.2.zip
Processed data (1)E-MEXP-3469.processed.1.zip
Array designA-AFFY-160.adf.txt
Links