E-MEXP-310 - Transcription profiling of wheat L88-18 line vs CTRL_L8831endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination dpg)
Released on 5 October 2005, last updated on 2 May 2014
The high molecular weight (HMW) subunits of wheat glutenin are synthesised only in the starchy endosperm tissue of the developing wheat grain. The transcriptomes of the lines L88-18 and L88-31 (Lawrence, G.J., Macritchie, F. & Wrigley, C.W. Dough and baking quality of wheat lines in glutenin subunits controlled by Glu-A1, Glu-B1 and Glu-D1 loci. J. Cereal. Sci. 7,109-112 (1988)) coming from the same cross were compared. Transcriptomes analysis was performed in endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination –dpg). Each of the transcriptome comparisons was performed using three biological replicates (i.e. per line/tissue /developmental stage selected). Hybridisations were performed in reverse dye labelling.
transcription profiling by array, genetic modification
Maria M Baudo <firstname.lastname@example.org>, Keith K Edwards, Melloney M McCluskey, Michael M Holdsworth, Peter P Shewry, Rebecca R Lyons, Stephen S Powers