E-MEXP-310 - Transcription profiling of wheat L88-18 line vs CTRL_L8831endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination dpg)

Status
Released on 5 October 2005, last updated on 2 May 2014
Organism
Triticum aestivum
Samples (18)
Array (1)
Protocols (11)
Description
The high molecular weight (HMW) subunits of wheat glutenin are synthesised only in the starchy endosperm tissue of the developing wheat grain. The transcriptomes of the lines L88-18 and L88-31 (Lawrence, G.J., Macritchie, F. & Wrigley, C.W. Dough and baking quality of wheat lines in glutenin subunits controlled by Glu-A1, Glu-B1 and Glu-D1 loci. J. Cereal. Sci. 7,109-112 (1988)) coming from the same cross were compared. Transcriptomes analysis was performed in endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination –dpg). Each of the transcriptome comparisons was performed using three biological replicates (i.e. per line/tissue /developmental stage selected). Hybridisations were performed in reverse dye labelling.
Experiment types
transcription profiling by array, genetic modification
Contacts
Maria M Baudo <marcela.baudo@bbsrc.ac.uk>, Keith K Edwards, Melloney M McCluskey, Michael M Holdsworth, Peter P Shewry, Rebecca R Lyons, Stephen S Powers
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-310.idf.txt
Sample and data relationshipE-MEXP-310.sdrf.txt
Raw data (1)E-MEXP-310.raw.1.zip
Processed data (1)E-MEXP-310.processed.1.zip
Array designA-MEXP-177.adf.txt
Links