E-MEXP-309 - Transcription profiling of wheat GF- TRANS_B102,1-1 vs Conv.bred_L88-18 transgenic lines endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination ?dpg)

Status
Released on 5 October 2005, last updated on 2 May 2014
Organism
Triticum aestivum
Samples (18)
Array (1)
Protocols (11)
Description
The high molecular weight (HMW) subunits of wheat glutenin are synthesised only in the starchy endosperm tissue of the developing wheat grain. We compared the expressed genomes of the transgenic wheat line B102,1-1 (Rooke et al. Transgene inheritance, segregation and expression in bread wheat. Euphytica 129, 301-309 (2003)). Both lines were shown to express the HMW-GS Ax1 gene (Halford, N.G. et al. Analysis of HMW glutenin subunits encoded bychromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality. Theor Appl Genet 83, 373-378 (1992).) to the expressed genome of conventionally bred wheat line L88-18 (Lawrence et al. Dough and baking quality of wheat lines in glutenin subunits controlled by Glu-A1, Glu-B1 and Glu-D1 loci. J. Cereal. Sci. 7,109-112 (1988)) which results in the same effects on traits. Transcriptomes comparison analysis was performed in endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination –dpg), respectively. Each of the transcriptome comparisons was performed using three biological replicates (i.e. per line/tissue /developmental stage selected). Hybridisations were performed in reverse dye labelling.Exceptionally, biological replica 2 was only performed for B102,1-1 (green)/L88-18 (red) labelling and not swap
Experiment types
transcription profiling by array, genetic modification
Contacts
Maria M Baudo <marcela.baudo@bbsrc.ac.uk>, Keith K Edwards, Melloney M Mc Cluskey, Michael M Holdsworth, Peter P Shewry, Rebecca R Lyons, Stephen S Powers
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-309.idf.txt
Sample and data relationshipE-MEXP-309.sdrf.txt
Raw data (1)E-MEXP-309.raw.1.zip
Processed data (1)E-MEXP-309.processed.1.zip
Array designA-MEXP-177.adf.txt
Links