E-MEXP-308 - Transcription profiling of wheat GF- B1118-8-4 vs B1355-4-2 transgenic lines endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination ?dpg)

Status
Released on 5 October 2005, last updated on 2 May 2014
Organism
Triticum aestivum
Samples (18)
Array (1)
Protocols (11)
Description
Transgenic cadenza lines express five HMW subunits encoded by:Glu-B1 (14, 15) and Glu D1 (5, 10) and transgene Glu-A1 (Ax1). Transcriptomes of the wheat line B1118-8-4(6) (produced by co transformation with two plasmids: one carried the transgene HMW-GS 1Ax1and other the bar and gus gene sequences) and wheat line B1355-4-2(18) (generated by co transformation with the ‘clean’ fragments of the HMW-GS 1Ax1 transgene and the bar gene sequence) were compared. Transcriptomes analysis was performed in endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination –dpg). Each of the transcriptome comparisons was performed using three biological replicates (i.e. per line/tissue /developmental stage selected). Hybridisations were performed in reverse dye labelling
Experiment types
transcription profiling by array, genetic modification
Contact
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-308.idf.txt
Sample and data relationshipE-MEXP-308.sdrf.txt
Raw data (1)E-MEXP-308.raw.1.zip
Processed data (1)E-MEXP-308.processed.1.zip
Array designA-MEXP-177.adf.txt
Links