E-MEXP-3059 - Transcription profiling by array of Treponema denticola grown with cell-cell contact with other red complex organisms
Released on 10 August 2013, last updated on 3 June 2014
We utilized T. denticola as a model red complex organism to help determine whether red complex organisms can respond to one another upon cell-cell contact in mixed culture and the types of genes involved in such responses. We extracted RNA from combinations of T. denticola with either other P. intermedia, S. sanguinis, S. gordonii, F. nucleatum, P. gingivalis, or T. forsythia that had been centrifuged to induce cell contact, and identified the numbers and types of genes that were differentially regulated using microarrays.
transcription profiling by array, co-expression, growth condition design, in vitro