E-MEXP-296 - Transcription profiling of two human cellular models of erythroid differentiation: K562 erythroid-leukemia cells treated with 1uM arabinofuranosyl-cytosine (AraC), and CD34+ stem cells treated with 7U/ml erythropoietin

Status
Released on 31 December 2006, last updated on 12 October 2011
Organism
Homo sapiens
Samples (9)
Array (1)
Protocols (11)
Description
In this study we investigated two cellular models of erythroid differentiation: K562 erythroid-leukemia cells treated with 1uM arabinofuranosyl-cytosine (AraC), and CD34+ stem cells treated with 7U/ml erythropoietin. Time course experiments were performed in both analysed models. Total RNAs were isolated and used for probe synthesis and hybridization on Affymetrix human U133A arrays. We compared the two models of erythropoiesis regarding their functional similarity. Furthermore, we identified differentially expressed proteins in each model, assigned gene-ontology annotations and ordered them into clusters. Finally, we investigated which pathways were affected during the differentiation processes using GenMapp.
Experiment types
transcription profiling by array, development or differentiation, time series
Contacts
Stefan Wolfl <wolfl@uni-hd.de>, Angelika A Mueller, Larissa Pusch, Sotirios Ziagos, Stefan S Knoth, Torsten Kroll
Citation
Evaluation of in-vitro models for erythropoiesis using gene expression. Stefan Knoth; Angelika Mueller; Larissa Pusch; Sotirios Ziagos; Torsten Kroll; Stefan Wolfl.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-296.idf.txt
Sample and data relationshipE-MEXP-296.sdrf.txt
Raw data (1)E-MEXP-296.raw.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-MEXP-296.eSet.r
Links