E-MEXP-295 - RNAi knock down of four Argonaute paralogs (AGO1, AGO2, PIWI and Aubergine) and the microRNA-processing enzyme Drosha in Drosophila

Status
Released on 20 November 2006, last updated on 2 May 2014
Organism
Drosophila melanogaster
Samples (25)
Array (1)
Protocols (10)
Description
RNA silencing pathways are conserved gene regulation mechanisms that lead to degradation, translational repression or transcriptional silencing of target-transcripts selected based on complementarity with small RNA molecules. The fraction of the genome regulated by these pathways has not been established. Argonaute proteins play fundamental roles in RNA silencing. To identify their physiological targets at the genomic level, we examined expression profiles in Drosophila cells depleted of 4 Argonaute paralogs (i.e. AGO1, AGO2, PIWI or Aubergine). We also profiled cells depleted of the microRNA (miRNA)-processing enzyme Drosha.
Experiment types
RNAi profiling by array, cellular modification
Contacts
Jan Rehwinkel <Jan.Rehwinkel@cancer.org.uk>, Alexander A Stark, Elisa E Izaurralde, Pavel P Natalin, Steven S Cohen
Citation
Genome wide analysis of mRNAs regulated by Drosha and Argonaute proteins in Drosophila melanogaster. Jan Rehwinkel; Pavel Natalin; Alexander Stark; Steven Cohen; Elisa Izaurralde.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-295.idf.txt
Sample and data relationshipE-MEXP-295.sdrf.txt
Raw data (3)E-MEXP-295.raw.1.zip, E-MEXP-295.raw.2.zip, E-MEXP-295.raw.3.zip
Processed data (1)E-MEXP-295.processed.1.zip
Array designA-AFFY-35.adf.txt
R ExpressionSetE-MEXP-295.eSet.r
Links