E-MEXP-285 - Transcription profiling by array of Arabidopsis pollen grains, flowers, leaves, siliques and whole seedlings

Status
Released on 1 June 2005, last updated on 2 May 2014
Organism
Arabidopsis thaliana
Samples (10)
Array (1)
Protocols (10)
Description
Upon germination, pollen forms a tube that elongates dramatically through female tissues in order to reach and fertilize ovules. While essential for the life cycle of higher plants, the genetic basis underlying most of the process is not well understood. We used Affymetrix Arabidopsis ATH1 Genome Arrays covering more than 80% of the Arabidopsis genome to compare transcriptomes of cell-sorted, hydrated pollen grains with those of flowers, leaves, seedlings and siliques (all samples with duplicates). This comparison revealed that pollen expresses a reduced set of genes with increased proportions of enriched and selectively-expressed transcripts. Relative gene ontology (GO) category representations in pollen and vegetative tissues revealed a functional skew of the pollen transcriptome towards signaling, vesicle transport and cytoskeleton, suggestive of a commitment towards germination and tube growth. Relative gene ontology (GO) category representations in pollen and vegetative tissues reveal a functional skew of the pollen transcriptome towards signaling, vesicle transport and cytoskeleton, suggestive of a commitment towards germination and tube growth. Relative gene ontology (GO) category representations in pollen and vegetative tissues reveal a functional skew of the pollen transcriptome towards signaling, vesicle transport and cytoskeleton, suggestive of a commitment towards germination and tube growth. Gene family and pathway analysis allowed formulation of novel hypotheses for the role of non-classical MADS-box genes, small RNA pathways and cell cycle components in pollen.
Experiment types
transcription profiling by array, organism part comparison
Contacts
Jörg D Becker <jbecker@igc.gulbenkian.pt>, Cristina Pina, Francisco Pinto, José A Feijó
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-285.idf.txt
Sample and data relationshipE-MEXP-285.sdrf.txt
Raw data (1)E-MEXP-285.raw.1.zip
Processed data (1)E-MEXP-285.processed.1.zip
Array designA-AFFY-2.adf.txt
R ExpressionSetE-MEXP-285.eSet.r
Links