6 protocols
AccessionNameType
P-AFFY-6
feature_extraction
Title: Affymetrix CEL analysis. Description:
P-AFFY-2
labeling
Affymetrix:Protocol:Hybridization-Midi_Euk2v3
hybridization
P-MTAB-14473
grow
Drosophila larvae were grown on standard corn meal agar at 25 C.
(Parameters: time unit = seconds, temperature unit = C)
P-MTAB-14472
bioassay_data_transformation
Raw data was normalized using ExpressionFileCreator module of GenePattern at Broad Institute. Quantile normalization with background correction was done following robust multi-array average (RMA) method.
P-MTAB-14471
nucleic_acid_extraction
Total RNA from wing imaginal discs of control and test genotypes were isolated using Qiagen RNeasy columns. Quality of RNA was ascertained using Agilent Bioanalyzer.
(Parameters: Extracted product = total_RNA, Amplification = none)