E-MEXP-2753 - Transcription profiling by array of neoplastically transformed lgl mosaic wing imaginal disc epithelium of Drosophila melanogaster

Status
Released on 8 June 2011, last updated on 2 May 2014
Organism
Drosophila melanogaster
Samples (6)
Array (1)
Protocols (6)
Description
In Drosophila, homozygosity for tumor suppressors, like lethal giant larvae, lgl, results in neoplastic transformation of imaginal disc epithelium - characterized by loss of their apico-basal polarity, unrestricted growth and invasion into neighboring tissues/organs. In genetic mosaics, however, lgl mutant clones are eliminated shortly after their initiation by cell competition. On the other hand, lgl mutant clones generated in Minute (M) genetic background, which compromises cell competition from the neighboring wild type cells, display clonal progression and tissue invasion. To gain insight into the molecular players of fly tumorigensis we undertook genome-wide transcriptional profiling of neoplastically transformed mosaic wing imaginal discs where lgl- clones were induced in M genetic background. Mosaic wing imaginal discs carrying wild type (lgl+) clones, also generated in M genetic background, served as controls. Transcriptional profile revealed up-regulation of a number of signaling pathways, such as Hippo, Wnt, TGF-beta or EGFR. Transcriptional profile of lgl- mosaic imaginal discs therefore provides a useful resource for identification of genes/pathways that are functionally relevant for carcinogenesis
Experiment types
transcription profiling by array, co-expression, genetic modification, in vivo, individual genetic characteristics
Contact
Citation
Epithelial neoplasia in Drosophila entails switch to primitive cell states. Sumbul J. Khan; Anjali Bajpai; Mohammad Atif Alam; Ram P. Gupta; Sneh Harsh; Ravi K. Pandey; Surbhi Goel-Bhattacharya; Aditi Nigam; Arati Mishra; Pradip Sinha. Proc Natl Acad Sci U S A 110(24):E2163 (2013), Europe PMC 23708122
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-2753.idf.txt
Sample and data relationshipE-MEXP-2753.sdrf.txt
Raw data (1)E-MEXP-2753.raw.1.zip
Processed data (1)E-MEXP-2753.processed.1.zip
Array designA-AFFY-35.adf.txt
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