E-MEXP-2646 - Transcription profiling of honey bee queens treated with carbon dioxide and physical manipulation vs virgin controls

Status
Submitted on 26 March 2010, released on 30 January 2011, last updated on 2 May 2014
Organism
Apis mellifera
Samples (15)
Array (1)
Protocols (4)
Description
Mating is a complex process that causes many behavioral and physiological changes, but the factors triggering these changes and the underlying molecular processes are not well characterized. Honey bee queens provide a convenient system for dissecting these factors (e.g., physical manipulation, insemination volume, insemination substance) via instrumental insemination. We examined the effects of carbon dioxide (CO2), a commonly used anesthetic in instrumental insemination that causes changes similar to those observed after mating, and physical manipulation, which presumably mimics the act of copulation, on the brain transcriptional changes in honey bee queens. We found significant gene overlap between our study and previous mating studies in honey bee queens and Drosophila. This suggests that molecular pathways regulating the mating process are conserved across different mating regimes of honey bees as well as across insect orders.
Experiment types
transcription profiling by array, co-expression, dye swap, innate behavior, loop
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-2646.idf.txt
Sample and data relationshipE-MEXP-2646.sdrf.txt
Raw data (1)E-MEXP-2646.raw.1.zip
Processed data (1)E-MEXP-2646.processed.1.zip
Array designA-MEXP-755.adf.txt
Links