E-MEXP-2429 - Transcription profiling of yeast strains lacking Met4 or Met4 cofactors at time points after sulfur limitation

Status
Submitted on 27 October 2009, released on 30 November 2009, last updated on 2 May 2014
Organism
Saccharomyces cerevisiae
Samples (39)
Array (1)
Protocols (6)
Description
All yeast strains used in this study (Table 1) are in the W303 background (ade2-1 can1-100, his3-1,15 leu2-3,112 trp1-1 ura3). For sulfur limitation microarray studies, WT, met4 delete, met31 delete met32 delete, cbf1 delete, and met28 delete strains were grown in minimal B-media [see Cherest, H., and Surdin-Kerjan, Y. (1992). Genetic analysis of a new mutation conferring cysteine auxotrophy in Saccharomyces cerevisiae: updating of the sulfur metabolism pathway. Genetics 130, p51-58 for B-media composition] supplemented with 0.5mM methionine as the sole sulfur source. An aliquot of cells was harvested for a t=0 time point while the remainder were filtered through a .22um Stericup filter (Millipore), then washed and resuspended in pre-warmed (30 C) B-media lacking any source of sulfur. Cells were harvested after 20, 40, and 80 minutes.
Experiment types
transcription profiling by array, co-expression, growth condition, in vivo, strain or line, time series
Contact
Citation
Systematic dissection of combinatorial control by the Met4 transcriptional complex.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-2429.idf.txt
Sample and data relationshipE-MEXP-2429.sdrf.txt
Raw data (1)E-MEXP-2429.raw.1.zip
Processed data (1)E-MEXP-2429.processed.1.zip
Array designA-MEXP-1729.adf.txt
R ExpressionSetE-MEXP-2429.eSet.r
Links