E-MEXP-2300 - Transcription profiling of Arabidopsis thaliana ematode induced early giant cells and normalvascular root cells.
Submitted on 9 August 2009, released on 27 July 2010, last updated on 10 June 2011
Giant cells (GCs)formed by meloidogyne javanica 3 days post infection were isolated by microaspiration from the Arabidopsis Col0 galls. Three independent biological replicates were used for hybridisation. The controls for comparisons were vascular cylinder tissues from uninfected root segments at equivalent positions to the galls in the infected roots. RNA extracted directly from either the controls or the GCs were amplified two times with a RNA polimerase linear amplification based approach
transcription profiling by array, cell type comparison, co-expression, dye swap
Early transcriptomic events in microdissected Arabidopsis nematode-induced giant cells.