E-MEXP-202 - RNAi knock down of known nonsense-mediated mRNA decay (NMD) factors in Drosophila cells

Status
Released on 1 November 2005, last updated on 2 May 2014
Organism
Drosophila melanogaster
Samples (16)
Array (1)
Protocols (7)
Description
All eukaryotes studied to date have the capacity to detect and degrade mRNAs harboring premature translation termination codons (PTCs) in a process called nonsense-mediated mRNA decay (NMD) (reviewed in Wagner E & Lykke-Andersen J, 2002). This surveillance system allows the cell to prevent the expression of potentially harmful truncated proteins. trans-acting factors required for NMD in Drosophila are the proteins UPF1, UPF2, UPF3, SMG-1, SMG-5 and SMG-6. To identify mRNAs naturally regulated by NMD, we analyzed expression profiles in Drosophila cells RNAi-depleted of known NMD factors using high-density oligonucleotide arrays.
Experiment types
RNAi profiling by array, cellular modification
Contact
Citation
Nonsense-mediated mRNA decay factors act in concert to regulate common mRNA targets. Rehwinkel J, Letunic I, Raes J, Bork P, Izaurralde E.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-202.idf.txt
Sample and data relationshipE-MEXP-202.sdrf.txt
Raw data (1)E-MEXP-202.raw.1.zip
Processed data (1)E-MEXP-202.processed.1.zip
Array designA-AFFY-17.adf.txt
R ExpressionSetE-MEXP-202.eSet.r
Links