E-MEXP-1559 - Transcription profiling of rat hair and supporting cells from the cristae ampullaris in the inner ear

Status
Released on 1 May 2008, last updated on 2 May 2014
Organism
Rattus norvegicus
Samples (4)
Array (1)
Protocols (4)
Description
Using laser capture microdisection, the more superficial layer of the cristae ampullaris sensory epithelium encompassing types I and II hair cells, and the supporting cell cytoplasm interspersed between them was captured in the first pass, and the underlying supporting cell bodies resting on the basal lamina were captured in a second pass. Individual hair cells were identified under light microscopy at 200X magnification by their stereociliary tuft and the calyceal nerve ending surrounding them and the presence of their nucleus in the outermost portion of the epithelia.
Experiment types
transcription profiling by array, cell type comparison, co-expression, transcript identification
Contact
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-1559.idf.txt
Sample and data relationshipE-MEXP-1559.sdrf.txt
Processed data (1)E-MEXP-1559.processed.1.zip
Array designA-GEHB-4.adf.txt
Links