E-MEXP-1559 - Transcription profiling of rat hair and supporting cells from the cristae ampullaris in the inner ear
Released on 1 May 2008, last updated on 2 May 2014
Using laser capture microdisection, the more superficial layer of the cristae ampullaris sensory epithelium encompassing types I and II hair cells, and the supporting cell cytoplasm interspersed between them was captured in the first pass, and the underlying supporting cell bodies resting on the basal lamina were captured in a second pass. Individual hair cells were identified under light microscopy at 200X magnification by their stereociliary tuft and the calyceal nerve ending surrounding them and the presence of their nucleus in the outermost portion of the epithelia.
transcription profiling by array, cell type comparison, co-expression, transcript identification