E-MEXP-1522 - Transcription profiling of human pre and pro B cells from patients with childhood acute lymphoblastic leukemia
Submitted on 20 March 2008, released on 1 April 2008, last updated on 2 May 2014
This data was generated as part of a larger study in to the leukemic stem cell potential of sorted childhood ALL populations. This work has been submitted for publication with the following summary. "We examined the leukemic stem cell potential of blasts at different stages of maturation in childhood acute lymphoblastic leukemia. Human leukemic bone marrow was transplanted intrafemorally onto NOD/scid mice. Cells sorted using the B precursor differentiation markers CD19, CD20 and CD34 were isolated from patient samples and engrafted mice before serial transplantation onto primary or subsequent (up to quaternary) recipients. Surprisingly, blasts representative of all the different maturational stages were able to reconstitute and re-establish the complete leukemic phenotype in vivo. Sorted blast populations mirrored normal B precursor cells with transcription of a number of stage appropriate genes. These observations lead to a new model for leukemia-propagating stem cells in childhood ALL." Diagnosis bone marrow specimens from children with acute lymphoblastic leukemia were FACS sorted into populations with surface expression CD34+CD19+ and CD34-CD19+. Expression analysis was performed to assess the developmental state of these two blast populations. These initial data have not been confirmed by RT PCR and should be viewed as preliminary. We are continuing with our work to further assess the transcriptional status of these populations.
transcription profiling by array, all pairs, co-expression, development or differentiation
In childhood acute lymphoblastic leukemia, blasts at different stages of immunophenotypic maturation have stem cell properties. Christoph le Viseur, Marc Hotfilder, Simon Bomken, Kerrie Wilson, Silja Röttgers, André Schrauder, Annegret Rosemann, Julie Irving, Ronald Stam, Leonard D. Shultz, Jochen Harbott, Heribert Jürgens, Martin Schrappe , Rob Pieters, Josef Vormoor. Cancer Cell (2008)