E-MEXP-1498 - Transcription profiling of yeast protrotophic laboratory strain CEN.PK113-7D grown under glutamine or ammonia limited conditions

Status
Released on 1 June 2008, last updated on 2 May 2014
Organism
Saccharomyces cerevisiae
Samples (4)
Array (1)
Protocols (9)
Description
The protrotophic laboratory strain CEN.PK113-7D (MAT a) was grown in laboratory fermentors with a working volume of 1 litre at dilution rate (D) of 0.20 per hour (in duplicate for each nitrogen (glutamine and ammonium) limited condition). After cultivation for few hundred generations in ammonium, samples from 2 continuous cultures were taken for array analysis. After cultivation for few hundred generations in glutamine, one evolved strain was picked and cultivated in 2 biological replicate chemostats until steady state was reached, and samples for array analysis were collected. All collected cell samples were cooled below 2 degree C within ten seconds by mixing 40% sample and 60% crushed ice.
Experiment types
transcription profiling by array, co-expression, growth condition, reference
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-1498.idf.txt
Sample and data relationshipE-MEXP-1498.sdrf.txt
Raw data (1)E-MEXP-1498.raw.1.zip
Processed data (1)E-MEXP-1498.processed.1.zip
Array designA-AFFY-27.adf.txt
R ExpressionSetE-MEXP-1498.eSet.r
Links