E-MEXP-1498 - Transcription profiling of yeast protrotophic laboratory strain CEN.PK113-7D grown under glutamine or ammonia limited conditions
Released on 1 June 2008, last updated on 2 May 2014
The protrotophic laboratory strain CEN.PK113-7D (MAT a) was grown in laboratory fermentors with a working volume of 1 litre at dilution rate (D) of 0.20 per hour (in duplicate for each nitrogen (glutamine and ammonium) limited condition). After cultivation for few hundred generations in ammonium, samples from 2 continuous cultures were taken for array analysis. After cultivation for few hundred generations in glutamine, one evolved strain was picked and cultivated in 2 biological replicate chemostats until steady state was reached, and samples for array analysis were collected. All collected cell samples were cooled below 2 degree C within ten seconds by mixing 40% sample and 60% crushed ice.
transcription profiling by array, co-expression, growth condition, reference