E-MEXP-1436 - Transcription profiling time series of Campylobacter jejuni reveals a stationary phase physiological switch
Submitted on 22 January 2008, released on 30 August 2008, last updated on 2 May 2014
Growth curves of C. jejuni NCTC11168 were performed in BHI broth at 42C with agitation at 150rpm.o. At 16, 20, 40, 46 and 54 hours of growth, aliquots were removed from the culture, and the bacteria were mixed with RNA stabilisation agent. RNA was then extracted from the stabilised pellets, and treated with DNase I to remove any contaminating residual genomic DNA. The integrity and quantity of the RNA was then confirmed on RNA Nano 6000 lab chips using an Agilent 2100 Bioanalyzer. The total RNA was reverse transcribed to amino-allyl dUTP labelled cDNA, and chemically coupled to either Cy3 or Cy5-NHS ester dyes prior to hybridisation to microarrays. Hybridisations were performed between the 16h and 20h, 20h and 40h, 40h and 46h, 46h and 54h and 16h and 54h (loop) samples. Hybrididsations from three independent growth curves were performed, and two dye reversal repeats were performed for each comparison, giving a total of 30 microarray hybridisations.
transcription profiling by array, co-expression, growth condition, loop, time series
etabolite and transcriptome analysis of Campylobacter jejuni in vitro growth reveals a stationary phase physiological switch. J.A. Wright, A.J. Grant, D. Hurd, M. Harrison, E. Guccione, D.J. Kelly, D.J. Maskell. Mol Microbiol