E-MEXP-1435 - Transcription profiling of Plasmodium falciparum synchronized blood stages exposed to lethal doses of artesunate
Submitted on 22 January 2008, released on 1 June 2008, last updated on 2 May 2014
Each experiment consisted in an artesunate-free culture (control) and an artesunate treated culture. For each time point explored, cDNA from parasites with and without the drug were labeled with different cyanines, mixed and hybridized. To analyse dynamic transcriptome alterations, two pilot experiments were performed in which RNA was harvested at 90 and 180 minutes of incubation with the drug.
Since artesunate is active on ring stages as well as on older trophozoite stages, we decided to explore different time points along the erythrocytic cycle and search for genes affected at each time point. Therefore, five drug treatment experiments, staggered between 20 hours and 30 hours of parasite development, were performed for the comparison of drug versus no drug at 90 minutes and 3 hours.
Different expression levels in artesunate-exposed vs. control unexposed cultures could reflect the added effects of the actual response to artesunate together with a possible difference in growth rate/developmental stage. To identify the developmentally regulated genes in the artesunate-free control culture, the 3 hours RNA was hybridized against the time 0 RNA (3 control experiments).
transcription profiling by array, co-expression, compound treatment, dye swap, in vitro, replicate, time series
Dynamic RNA profiling in Plasmodium falciparum synchronized blood stages exposed to lethal doses of artesunate. Natalang O, Bischoff E, Deplaine G, Proux C, Dillies MA, Sismeiro O, Guigon G, Bonnefoy S, Patarapotikul J, Mercereau-Puijalon O, Coppée JY, David PH. :388 (2008), Europe PMC 18706115
Dynamic RNA profiling in Plasmodium falciparum synchronized blood stages exposed to lethal doses of artesunate. Onguma NATALANG, Emmanuel BISCHOFF, Guillaume DEPLAINE, Caroline PROUX, Marie-Agnes DILLIES, Odile SISMEIRO, Ghislaine GUIGON, Serge BONNEFOY, Jintana PATARAPOTIKUL, Odile MERCEREAU-PUIJALON, Jean-Yves COPPEE , Peter H. DAVID. Genome Biol (2008)