E-MEXP-1402 - RNAi knock down of endogenous genes and luciferase reporters in human HeLa cells to examine the effect of seed complement frequency on off-target signatures
Submitted on 4 January 2008, released on 15 March 2008, last updated on 2 May 2014
siRNAs were designed against both endogenous genes and luciferase reporters to examine the effect of seed complement frequency on off-target signatures as measured by Agilent Human 1A gene expression analysis.
RNAi profiling by array, cellular modification, co-expression, compound treatment, in vitro, reference, self vs self
Experimental validation of the importance of seed complement frequency to siRNA specificity. Anderson, Emily M.; Birmingham, Amanda; Baskerville, Scott; Reynolds, Angela; Maksimova, Elena; Leake, Devin; Fedorov, Yuriy; Karpilow, Jon; Khvorova, Anastasia. RNA 14(5):853 (2008), Europe PMC 18367722