E-MEXP-1402 - RNAi knock down of endogenous genes and luciferase reporters in human HeLa cells to examine the effect of seed complement frequency on off-target signatures

Status
Submitted on 4 January 2008, released on 15 March 2008, last updated on 2 May 2014
Organism
Homo sapiens
Samples (54)
Array (1)
Protocols (7)
Description
siRNAs were designed against both endogenous genes and luciferase reporters to examine the effect of seed complement frequency on off-target signatures as measured by Agilent Human 1A gene expression analysis.
Experiment types
RNAi profiling by array, cellular modification, co-expression, compound treatment, in vitro, reference, self vs self
Contact
Citation
Experimental validation of the importance of seed complement frequency to siRNA specificity. Anderson, Emily M.; Birmingham, Amanda; Baskerville, Scott; Reynolds, Angela; Maksimova, Elena; Leake, Devin; Fedorov, Yuriy; Karpilow, Jon; Khvorova, Anastasia. RNA 14(5):853 (2008), Europe PMC 18367722
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-1402.idf.txt
Sample and data relationshipE-MEXP-1402.sdrf.txt
Raw data (2)E-MEXP-1402.raw.1.zip, E-MEXP-1402.raw.2.zip
Array designA-AGIL-9.adf.txt
Links