E-MEXP-1385 - Transcription profiling of wild type and Foxc-1 knock-out mouse embryos
Submitted on 12 December 2007, released on 14 December 2007, last updated on 2 May 2014
Wild-type vs. mutant on candidate cDNA array: Total RNA was extracted from the whole heads of 13.5 wild-type and mutant embryos using TriPure reagent (Roche) and purified using TriPure reagent was purified using the RNeasy Mini kit (Qiagen). Labelled cDNA was generated from RNA extracted from six pooled wild-type and six pooled mutant samples. Labelled cDNA was synthesised using the 3DNA Array 50 kit (Genisphere) and hybridised to the candidate cDNA array. For the oligo array, labelled cDNA was generated from RNA extracted from six pooled wild type and six pooled mutant samples. Labelled cDNA was generated using the amino-alyll labeling method.
transcription profiling by array, co-expression, dye swap, transcript identification