E-MEXP-1385 - Transcription profiling of wild type and Foxc-1 knock-out mouse embryos

Status
Submitted on 12 December 2007, released on 14 December 2007, last updated on 2 May 2014
Organism
Mus musculus
Samples (12)
Arrays (2)
Protocols (11)
Description
Wild-type vs. mutant on candidate cDNA array: Total RNA was extracted from the whole heads of 13.5 wild-type and mutant embryos using TriPure reagent (Roche) and purified using TriPure reagent was purified using the RNeasy Mini kit (Qiagen). Labelled cDNA was generated from RNA extracted from six pooled wild-type and six pooled mutant samples. Labelled cDNA was synthesised using the 3DNA Array 50 kit (Genisphere) and hybridised to the candidate cDNA array. For the oligo array, labelled cDNA was generated from RNA extracted from six pooled wild type and six pooled mutant samples. Labelled cDNA was generated using the amino-alyll labeling method.
Experiment types
transcription profiling by array, co-expression, dye swap, transcript identification
Contact
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-1385.idf.txt
Sample and data relationshipE-MEXP-1385.sdrf.txt
Raw data (1)E-MEXP-1385.raw.1.zip
Array designsA-MEXP-238.adf.txt, A-MEXP-240.adf.txt
Links