E-MEXP-1247 - Transcription profiling of parasitic fungus Monacrosporium haptotylum during infection of nematode C. elegans
Submitted on 7 September 2007, released on 1 June 2008, last updated on 10 June 2011
Caenorhabditis elegans, Monacrosporium haptotylum
This investigation was designed to follow the parasitic fungus Monacrosporium haptotylum during infection of the nematode Caenorhabditis elegans. M. haptotylum traps nematodes using a spherical structure called knob, which develops on the apex of a hyphal branch. The advantage of using this species is that the unicellular knobs can be separated from the mycelium that has been grown in liquid culture. The isolated knobs retain the function as infection structures. The cDNA microarray contained 2,778 M. haptotylum and 588 C. elegans gene reporters. The experiments was designed as a time course study including infected and non-infected tissue, over 24 hours which involves stages of adhesion, penetration and digestion of the infected nematodes.
transcription profiling by array, co-expression, disease state, dye swap, loop, pathogenicity, reference, replicate, time series
Paralysis of nematodes: shifts in the transcriptome of the nematode-trapping fungus Monacrosporium haptotylum during infection of Caenorhabditis elegans. Csaba Fekete, Margareta Tholander, Balaji Rajashekar, Dag Ahrén, Eva Friman, Tomas Johansson and Anders Tunlid. Environ Microbiol (2007)