E-MEXP-1190 - Transcription profiling time series of kidney, liver and spleen from three strains of mice infected with Trypanosoma congolense to investigate strain differences in susceptibility
Submitted on 27 July 2007, released on 21 August 2007, last updated on 27 March 2012
In this experiment, mice from 3 strains A/J, Balb C and C57Bl6 were infected with Trypanosoma Congolense, IL1180 clone, African sleeping sickness, a disease which affects cattle in sub-saharan Africa. These mouse strains were chosen because they are a model for tolerance to infection in cattle, with A/J and Balb B being highly susceptible, and C57Bl6 being somewhat more tolerant to infection. Three tissues Kidney Liver and Spleen were harvested from cohorts at various timepoints: 0 (naive), 3, 7, 9 and 17 days post infection. Each condition thus comprises: Time, Tissue, Strain. For each condition, RNA extracts from individuals were pooled (5 per pool) prior to hybridisation.
transcription profiling by array, co-expression, disease state, organism part comparison, replicate, strain or line, time series
A systematic strategy for large-scale analysis of genotype phenotype correlations: identification of candidate genes involved in African trypanosomiasis. Fisher, Paul; Hedeler, Cornelia; Wolstencroft, Katherine; Hulme, Helen; Noyes, Harry; Kemp, Stephen; Stevens, Robert; Brass, Andrew. Nucleic Acids Res 35(16):5625 (2007), Europe PMC 17709344