E-MEXP-1119 - Transcription profiling of Streptomyces coelicolor strain M653 in a time series after ppGpp synthesis activation by thiostrepton
Submitted on 22 May 2007, released on 1 August 2007, last updated on 10 June 2011
ppGpp synthesis was activated in exponentially growing cells of Streptomyces coelicolor in the absence of amino acid starvation by using a strain (M653 [DrelA tipAp::relA(1.46kb)]) that expresses a truncated portion of relA under the control of a thiostrepton-inducible promoter. Samples were harvested at 30 min intervals following induction for comparison to a control set of samples from aliquots of the same cultures that were not induced.
transcription profiling by array, co-expression, in vivo, stimulus or stress, time series
The global role of ppGpp in morphological differentiation and antibiotic production in Streptomyces coelicolor A3(2). Andrew Hesketh, Joan Chen, Jamie Ryding, Sherman Chang, Mervyn Bibb. Genome Biol