E-MEXP-1104 - Transcription profiling of yeast TOP1 null strain bearing plasmids that express wild-type yeast Top1p or an inactive Y727F mutant enzyme and treated with camptothecin
Submitted on 9 May 2007, released on 1 June 2008, last updated on 2 May 2014
To establish the effect of CPT in the regulation of global transcription in S. cerevisiae, we have used a yeast TOP1 null strain, JEL1Δtop1, bearing a low-copy number plasmid that expresses, under the control of the yeast TOP1 promoter, either a wild-type (wt) yeast Top1p (pCC10) or an inactive Y727F mutant enzyme (pAR7).We determined global transcript levels upon CPT treatments of JEL1Δtop1 cells expressing either a yeast wt or Y727F mutant Top1p.
transcription profiling by array, co-expression, compound treatment, in vitro
title. author1. other[Molecular Pharmacology]