E-MEXP-1104 - Transcription profiling of yeast TOP1 null strain bearing plasmids that express wild-type yeast Top1p or an inactive Y727F mutant enzyme and treated with camptothecin

Status
Submitted on 9 May 2007, released on 1 June 2008, last updated on 2 May 2014
Organism
Saccharomyces cerevisiae
Samples (4)
Array (1)
Protocols (9)
Description
To establish the effect of CPT in the regulation of global transcription in S. cerevisiae, we have used a yeast TOP1 null strain, JEL1Δtop1, bearing a low-copy number plasmid that expresses, under the control of the yeast TOP1 promoter, either a wild-type (wt) yeast Top1p (pCC10) or an inactive Y727F mutant enzyme (pAR7).We determined global transcript levels upon CPT treatments of JEL1Δtop1 cells expressing either a yeast wt or Y727F mutant Top1p.
Experiment types
transcription profiling by array, co-expression, compound treatment, in vitro
Contact
Citation
title. author1. other[Molecular Pharmacology] 
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-1104.idf.txt
Sample and data relationshipE-MEXP-1104.sdrf.txt
Raw data (1)E-MEXP-1104.raw.1.zip
Processed data (1)E-MEXP-1104.processed.1.zip
Array designA-MEXP-659.adf.txt
R ExpressionSetE-MEXP-1104.eSet.r
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