E-MAXD-24 - Transcription profiling of major osmoregulatory tissues of Anguilla anguilla in response to the sea water environment

Status
Released on 19 April 2008, last updated on 2 May 2014
Organism
Anguilla anguilla
Samples (1)
Array (1)
Protocols (19)
Description
The aim of this study was to use a microarray approach to screen the major osmoregulatory tissues of the eel for changes in gene expression as fish acclimate to the SW environment. The other species (few clones) were used to validate the specificity of arrays hybridizations : Of the 6144 clones spotted, 96 contained control cDNAs comprising either known eel genes implicated in osmoregulation and previously studied in our laboratory or coding regions of genes sequenced from other species (ie. Aspergillus nidulans, Arabidopsis thaliana, Pleuronectes flesus, Carcharhinus leucas, Scyliorhinus canicula, Squalus acanthias and Salmo salar). The labelled cDNA probes derived from the eel RNAs hybridised only with the spotted cDNAs from the eel clones indicating the stringency and probe specificities of the hybridisation conditions were appropriate.
Experiment types
transcription profiling by array, organism part comparison
Contacts
Citation
Transcriptomic approach to the study of osmoregulation in the European eel Anguilla anguilla. Kalujnaia S, McWilliam IS, Zaguinaiko VA, Feilen AL, Nicholson J, Hazon N, Cutler CP, Cramb G. Physiol Genomics 31(3):385-401 (2007), Europe PMC 17666525
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MAXD-24.idf.txt
Sample and data relationshipE-MAXD-24.sdrf.txt
Raw data (1)E-MAXD-24.raw.1.zip
Array designA-MAXD-13.adf.txt
R ExpressionSetE-MAXD-24.eSet.r
Links