E-MARS-20 - Transcription profiling by array for comparison of industrial Escherichia coli strains on growth, transcriptome and proteome level in batch cultivations to identify strain specific features (subexperiment K-12 platform)

Status
Released on 19 August 2013, last updated on 3 June 2014
Organism
Escherichia coli
Samples (9)
Array (1)
Protocols (7)
Description
The objective of the current study was to evaluate the performance of frequently used E. coli K-12 strains HMS174 and RV308 in the context of industrial cultivations. To meet this objective, we carried out batch cultivations with high-glucose media in computer-controlled bioreactors and applied transcriptome analysis to interpret phenotypic attributes at the molecular level. The resulting enhancement of knowledge about host physiology is expected to contribute to the acceleration of improvements and optimizations in related biotech processes.
Experiment types
transcription profiling by array, dye swap, reference, strain or line
Contacts
Citation
A Comparative Analysis of Industrial Escherichia coli K–12 and B Strains in High-Glucose Batch Cultivations on Process-, Transcriptome- and Proteome Level. Karoline Marisch, Karl Bayer, Theresa Scharl, Juergen Mairhofer, Peter M. Krempl, Karin Hummel, Ebrahim Razzazi-Fazeli, Gerald Striedner. PLoS One 8(8) (2013)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MARS-20.idf.txt
Sample and data relationshipE-MARS-20.sdrf.txt
Raw data (1)E-MARS-20.raw.1.zip
Processed data (1)E-MARS-20.processed.1.zip
Array designA-MARS-12.adf.txt
Links