E-MARS-16 - Transcription profiling of E. coli HMS174(DE3)(pET30aNproGFPmut3.1) to analyze cellular response to full induction with IPTG

Status
Released on 26 March 2010, last updated on 12 October 2011
Organism
Escherichia coli
Samples (18)
Array (1)
Protocols (7)
Description
This transcription profiling time course experiment was conducted in order to analyze the cellular response of the T7 RNA polymerase based E. coli expression system HMS174(DE3)(pET30aNproGFP) to a single pulse induction with isopropyl-beta-D-galactoside (IPTG). The used cell material was produced under well controlled and defined conditions in an exponential carbon limited fed-batch cultivation at a growth rate of 0.1 per hour. The applied amount of 20µmol IPTG per gram cell dry weight assured a fully induced system and high recombinant gene expression rates. Non induced cells sampled at the time point of induction were used as reference and compared to cells subsequently sampled during the production period (sampling frequency 1 hour)
Experiment types
transcription profiling by array, time series
Contacts
Citation
Exploratory and inferential analysis of gene cluster neighborhood graphs. Scharl T, Voglhuber I, Leisch F. BMC Bioinformatics 10:288 (2009), Europe PMC 19751523
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MARS-16.idf.txt
Sample and data relationshipE-MARS-16.sdrf.txt
Raw data (1)E-MARS-16.raw.1.zip
Array designA-MARS-10.adf.txt
Links