E-GEOD-9973 - Transcription profiling of human B-cells (BL41) for tc-, nt- and p-RNA half-life determination

Status
Submitted on 19 December 2007, released on 20 October 2009, last updated on 2 May 2014
Organism
Homo sapiens
Samples (9)
Array (1)
Protocols (6)
Description
RNA was labeled in BL41 cells by culturing cells for 60 min in media containing 100µM 4sU. Tc-RNA was separated into nt- and p-RNA. All three RNA subsets were subjected to microarray analysis. Only probe sets providing present calls in all RNA samples/subsets were included into the analysis; We used microarrays to determine half-lives of mRNAs expressed in BL41 cells Experiment Overall Design: RNA was labeled in BL41 cells by culturing cells for 60 min in media containing 100µM 4sU. Tc-RNA was separated into nt- and p-RNA. All three RNA subsets were subjected to microarray analysis.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-9973.idf.txt
Sample and data relationshipE-GEOD-9973.sdrf.txt
Raw data (1)E-GEOD-9973.raw.1.zip
Processed data (1)E-GEOD-9973.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-9973.eSet.r
Links