E-GEOD-972 - Transcription profiling of early development of neural crest stem cells and developing Schwann cells, from embryonic day 9.5 to postnatal day 0
Submitted on 16 January 2004, released on 13 December 2007, last updated on 27 March 2012
Time course of early development of peripheral nerve, from embryonic day 9.5 to postnatal day 0. Origin of samples: mouse embryos expressing green fluorescent protein (GFP) in neural crest stem cells and later glial cells under the control of the proteolipid protein gene (Plp) promoter. At E9.5 the trunk region was dissected out. At E12, E14, E16, E18 and P0 the sciatic nerve was taken. GFP-expressing cells were isolated by FACS sorting. Three or 4 separate batches of embryos analyzed at each stage.
transcription profiling by array, cell type comparison, co-expression, development or differentiation
Efficient isolation and gene expression profiling of small numbers of neural crest stem cells and developing Schwann cells. Johanna Buchstaller, Lukas Sommer, Matthias Bodmer, Reinhard Hoffmann, Ueli Suter, Ned Mantei. J Neurosci 24(10):2357-65 (2004)