E-GEOD-9531 - Transcription profiling by array of rhesus macaque tissue samples and a fibroblast cell line

Status
Released on 15 June 2008, last updated on 11 May 2012
Organism
Macaca mulatta
Samples (20)
Arrays (2)
Protocols (6)
Description
The primary goal of this study was to compare the performances of Rhesus Macaque Genome Array and Human Genome U133 Plus 2.0 Array with respect to the detection of differential expressions when rhesus macaque RNA extracts were labeled and hybridized. The secondary goal of this study was to investigate the effect of mismatch position on signal strength in Affymetrix GeneChips by examining naturally occurring mismatches between rhesus macaque transcripts and human probes from Human Genome U133 Plus 2.0 Array. The primary goal of this study was to compare the performances of Rhesus Macaque Genome Array and Human Genome U133 Plus 2.0 Array with respect to the detection of differential expressions when rhesus macaque RNA extracts were labeled and hybridized. The secondary goal of this study was to investigate the effect of mismatch position on signal strength in Affymetrix GeneChips by examining naturally occurring mismatches between rhesus macaque transcripts and human probes from Human Genome U133 Plus 2.0 Array. Keywords: cross hybridization Rhesus macaque RNA from five sources (immortalized fibroblasts, cerebral cortex, pancreas, testes and thymus) was divided into two sets of aliquots of equal amount. Samples from each of the five sources were labeled and hybridized with either Rhesus Macaque Genome Array or two Human Genome U133 Plus 2.0 Array. Rhesus macaque RNA from five sources (immortalized fibroblasts, cerebral cortex, pancreas, testes and thymus) was divided into two sets of aliquots of equal amount. Samples from each of the five sources were labeled and hybridized with either Rhesus Macaque Genome Array or two Human Genome U133 Plus 2.0 Array.
Experiment type
transcription profiling by array 
Contacts
Fenghai Duan <fduan@unmc.edu>, Eliot R Spindel, Robert B Norgren
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-9531.idf.txt
Sample and data relationshipE-GEOD-9531.sdrf.txt
Raw data (1)E-GEOD-9531.raw.1.zip
Processed data (1)E-GEOD-9531.processed.1.zip
Array designsA-AFFY-145.adf.txt, A-AFFY-44.adf.txt
R ExpressionSetE-GEOD-9531.eSet.r
Links