E-GEOD-923 - Transcription profiling of human lung epithelial cells post-infected with P aeruginosa strains

Status
Submitted on 19 December 2003, released on 28 September 2007, last updated on 27 March 2012
Organism
Homo sapiens
Samples (19)
Array (1)
Description
This series contains 5 groups of U133A arrays with targets from Calu-3 cell line post-infection with P aeruginosa strains. Calu-3 human lung epithelial cells were seeded onto 6-well plates and grown to about 80% confluency. Epithelial monolayers were infected with 108 CFUs per ml of each bacterial strain for 60 min and then washed extensively with phosphate-buffered saline. The cultures were subsequently incubated in fresh RPMI 1640 medium supplemented with 10% fetal bovine serum and 100 µg/ml gentamicin. After 6 h, total RNA from the cells was prepared with RNAzol and further purified with RNEasy for microarray hybridization. All arrays processed and globally scaled to 500 using MAS 5. CONTROL (4 arrays) - no infection; FRD1 (4 arrays); FRD1234 (3 arrays); FRD440 (4 arrays); FRD875 (4 arrays)
Experiment types
transcription profiling by array, disease state, individual genetic characteristics
Contact
Citation
Pseudomonas aeruginosa flagellin and alginate elicit very distinct gene expression patterns in airway epithelial cells: implications for cystic fibrosis disease. Laura M Cobb, Josyf C Mychaleckyj, Daniel J Wozniak, Yolanda S López-Boado. J Immunol 173(9):5659-70 (2004)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-923.idf.txt
Sample and data relationshipE-GEOD-923.sdrf.txt
Processed data (1)E-GEOD-923.processed.1.zip
Array designA-AFFY-33.adf.txt
Links