E-GEOD-8946 - Transcription profiling of mouse Creb1 abalated hippocampus fin a Crem knockout background treated with kainate
Submitted on 4 September 2007, released on 16 June 2008, last updated on 10 June 2011
Ablation of the Creb1 gene in forebrain neurons was performed using the Cre/loxP system, with the recombinase expressed from the Camk2alfa promoter. Mice were crossed into the Crem KO background to prevent compensation of CREB loss by CREM overexpression. Our goal was to analyze how loss of CREB will affect acitivity-regulated transcription induced by strong stimulation, i.e. kainate. Experiment Overall Design: Animals were injected i.p. with either 20 mg/kg kainate or saline and sacrificed after one hour by cervical dislocation. Expression profiling was performed using total RNA isolated from the hippocampus.
transcription profiling by array, unknown experiment type