E-GEOD-8944 - Transcription profiling of mouse hippocampus from animals with Creb1 abalated forebrains in a Crem1 knockout background

Status
Submitted on 4 September 2007, released on 16 June 2008, last updated on 10 June 2011
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (4)
Description
Ablation of the Creb1 gene in forebrain neurons was performed using the Cre/loxP system, with the recombinase expressed from the Camk2alfa promoter. Mice were crossed into the Crem KO background to prevent compensation of CREB loss by CREM overexpression. Our goal was to analyze how loss of CREB will affect acitivity-regulated transcription induced by strong stimulation, i.e. kainate. Experiment Overall Design: Animals were injected i.p. with either 20 mg/kg kainate or saline and sacrificed after one hour by cervical dislocation. Expression profiling was performed using total RNA isolated from the hippocampus.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-8944.idf.txt
Sample and data relationshipE-GEOD-8944.sdrf.txt
Processed data (1)E-GEOD-8944.processed.1.zip
Array designA-AFFY-6.adf.txt
Links