E-GEOD-8909 - Profiling differentially expressed earthworm genes in response to TNT, RDX, or TNT+RDX exposure.

Status
Submitted on 28 August 2007, released on 28 August 2008, last updated on 2 May 2014
Organism
Eisenia fetida
Samples (80)
Array (1)
Protocols (7)
Description
To understand molecular mechanisms of the joint effects of 2,4,6-trinitrotoluene (TNT) and hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), both widely used ordnance compounds, we constructed a microarray consisting of 4,032 cDNA isolated from the earthworm Eisenia fetida using the suppressive subtractive hybridization technique. Worms were exposed to TNT-, RDX-, or TNT+RDX-spiked soil for 28 days (TNT 50 mg/kg, RDX 30 mg/kg). Keywords: Combined toxicity of TNT and RDX to earthworm (Eisenia fetida) We analyzed 40 arrays for 4 treatments (control, TNT 50ppm, RDX 30ppm, TNT 50ppm + RDX 30ppm) with 5 biological replicates per treatment using an interwoven loop design.
Experiment type
transcription profiling by array 
Contacts
Ping Gong <ping.gong@us.army.mil>, Edward J Perkins, Xin Guan
Citation
MIAME
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