E-GEOD-8868 - Transcription profiling of mouse splenic and small intestine lamina propria macrophages

Status
Submitted on 24 August 2007, released on 16 June 2008, last updated on 2 May 2014
Organism
Mus musculus
Samples (4)
Array (1)
Protocols (7)
Description
The intestinal immune system must elicit robust immunity against harmful pathogens but restrain immune responses directed against commensal microbes and dietary antigens. The mechanisms that maintain this dichotomy are poorly understood. Here we describe a population of CD11b+F4/80+CD11c– macrophages in the lamina propria (LP) that express several anti-inflammatory molecules including interleukin 10 (IL-10), but little or no pro-inflammatory cytokines, even upon stimulation with Toll-like receptor (TLR) ligands. These macrophages induced, in a manner dependent on IL-10, retinoic acid and exogenous transforming growth factor-β, differentiation of FoxP3+ regulatory T cells. In contrast, LP CD11b+ dendritic cells elicited IL-17 production. This IL-17 production was suppressed by LP macrophages, indicating that a dynamic interplay between these subsets may influence the balance between immune activation and tolerance. Splenic or small intestine lamina propria CD11b+11c- cells were isolated for RNA extraction and hybridization on Affymetrix microarrays. We sought to determine the unique genetic profile of small intestine lamina propria CD11b+11c- cells. Experiment Overall Design: 4 samples analyzed, 2 spleen and 2 intestine
Experiment types
transcription profiling by array, unknown experiment type
Contact
Tim Denning
Citation
Lamina propria macrophages and dendritic cells differentially induce regulatory and interleukin 17-producing T cell responses. Timothy L Denning, Yi-chong Wang, Seema R Patel, Ifor R Williams, Bali Pulendran. Nat Immunol 8(10):1086-94 (2007)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-8868.idf.txt
Sample and data relationshipE-GEOD-8868.sdrf.txt
Raw data (1)E-GEOD-8868.raw.1.zip
Processed data (1)E-GEOD-8868.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-8868.eSet.r
Links