E-GEOD-8828 - Transcription profiling of mouse cancer stem cells in MMTVWnt-1 murine breast tumors

Submitted on 20 August 2007, released on 16 June 2008, last updated on 27 March 2012
Mus musculus
Samples (6)
Array (1)
Protocols (4)
In human breast cancers, a phenotypically distinct minority population of tumorigenic cancer (TG) cells (sometimes referred to as cancer stem cells) drives tumor growth when transplanted into immunodeficient mice. Our objective was to identify a mouse model of breast cancer stem cells that could have relevance to studying human breast cancer. To do so, we utilized breast tumors of the MMTVWnt-1 mice. MMTV-Wnt-1 breast tumors were harvested, dissociated into single cell suspensions, and FACS sorted on Thy1, CD24, and CD45. FACS sorted cells were then injected into recipient background FBV/NJ female mice. Thy1+CD24+ cancer cells, which constitute approximately 1-4% of tumor cells were highly enriched for cells capable of regenerating new tumors when compared to cells of the tumor that did not fit this profile (“Not Thy1+CD24+”). Resultant tumors were of the same phenotypic diversity as the original tumor and behaved in a similar manner when passaged. Microarray analysis comparing Thy1+CD24+ tumor cells to “Not Thy1+CD24+” cells identified a list of differentially expressed genes. Orthologs of these differentially expressed genes predicted survival of human breast cancer patients from two different study groups. These studies suggest that there is a cancer stem cell compartment in the MMTV-Wnt-1 murine breast tumor and that there is a clinical utility of this model for the study of cancer stem cells. Experiment Overall Design: Expression profling were performed on 3 tumorigenic and 3 non tumorigenic samples of MMTV-Wnt-1 breast tumors. A gene signature was derived by comparing the gene expressions of 3 tumorigenic samples with 3 nontumorigenic samples
Experiment types
transcription profiling by array, unknown experiment type
Isolation and molecular characterization of cancer stem cells in MMTV-Wnt-1 murine breast tumors. Robert W Cho, Xinhao Wang, Maximilian Diehn, Kerby Shedden, Grace Y Chen, Gavin Sherlock, Austin Gurney, John Lewicki, Michael F Clarke. Stem Cells 26(2):364-71 (2008)
Investigation descriptionE-GEOD-8828.idf.txt
Sample and data relationshipE-GEOD-8828.sdrf.txt
Raw data (1)E-GEOD-8828.raw.1.zip
Processed data (1)E-GEOD-8828.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-8828.eSet.r