E-GEOD-8818 - Transcription profiling of mouse intestinal crypts upon deletion of beta-catenin

Status
Submitted on 20 August 2007, released on 16 June 2008, last updated on 2 May 2014
Organism
Mus musculus
Samples (4)
Array (1)
Protocols (5)
Description
The Wnt signaling pathway is deregulated in over 90% of human colorectal cancers. β Catenin, the central signal transducer of the Wnt pathway, can directly modulate gene expression by interacting with transcription factors of the TCF/LEF-family. In the present study we investigate the role of Wnt signaling in the homeostasis of intestinal epithelium using tissue-specific, inducible beta-catenin gene ablation in adult mice. Block of Wnt/beta-catenin signaling resulted in rapid loss of transient-amplifying cells and crypt structures. Importantly, intestinal stem cells were induced to terminally differentiate upon deletion of beta-catenin resulting in a complete block of intestinal homeostasis and fatal loss of intestinal function. Transcriptional profiling of mutant crypt mRNA isolated by laser capture micro dissection confirmed those observations and allowed to identify genes potentially responsible for the functional preservation of intestinal stem cells. Experiment Overall Design: laser capture microdissection of intestinal crypts, control vs. beta-catenin mutant (2days after induction of deletion by tamoxifen), two rounds of amplification of mRNA
Experiment types
transcription profiling by array, unknown experiment type
Contact
Joerg Huelsken
Citation
Wnt/beta-catenin is essential for intestinal homeostasis and maintenance of intestinal stem cells. Tea Fevr, Sylvie Robine, Daniel Louvard, Joerg Huelsken. Mol Cell Biol 27(21):7551-9 (2007)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-8818.idf.txt
Sample and data relationshipE-GEOD-8818.sdrf.txt
Raw data (1)E-GEOD-8818.raw.1.zip
Processed data (1)E-GEOD-8818.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-8818.eSet.r
Links